Peptide microarrays coupled to machine learning reveal individual epitopes from human antibody responses with neutralizing capabilities against SARS-CoV-2기계 학습과 결합된 펩티드 마이크로어레이는 SARS-CoV-2에 대한 중화 기능을 가진 인간 항체 반응의 개별 에피토프를 나타냅니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료법, [키워드] anti-SARS-CoV-2 antibody Antibody binding assays binding epitope capture causative agent cellular coronavirus coronavirus SARS-CoV-2 Course COVID-19 detectable epitope Epitopes human antibody response IgA IgG IgM antibody immunoassays incubated infected with SARS-CoV-2 machine learning Microarray N-terminus negative control samples neutralization neutralization assays Neutralizing Neutralizing antibodies nine overlapping Patient peptide peptide arrays peptides positive protective effect Protective effects random Random forest response S protein SARS CoV SARS CoV-2 SARS-CoV-2 sera serology Serology. Specific structural protein structural proteins the disease the S protein vaccine candidate vaccine candidates Viral [DOI] 10.1080/22221751.2022.2057874 PMC 바로가기 [Article Type] Article
Preparation and pre-clinical evaluation of flagellin-adjuvanted NOM vaccine candidate formulated with Spike protein against SARS-CoV-2 in mouse modelArticle Published on 2022-10-012022-11-15 Journal: Microbial pathogenesis [Category] COVID19(2023년), SARS, 진단, [키워드] 3D structure acute respiratory syndrome Analysis anti-S Antigen Antisera bind Cell Cell-mediated immune responses chimeric China Cluster coronavirus cytokine develop docking effective elicit ELISA ENhance evaluated expressed flagellin groups humoral IFN-γ IgG IL-10 IL-17 IL-22 IL-6 immune response immunoinformatics indicated Infection Interaction mice Ni-NTA NOM protein ORF3a outbreak performed pneumonia case preparation promoted Protein purified raised receptor S protein Salmonella SARS-CoV-2 significantly increased spike spleen TLR5 TNF-α Total vaccination Vaccine vaccine candidate vaccine candidates vaccine. was selected were assessed Wuhan [DOI] 10.1016/j.micpath.2022.105736 PMC 바로가기
Innate Immune Response and Inflammasome Activation During SARS-CoV-2 InfectionArticle Published on 2022-10-012022-11-15 Journal: Inflammation [Category] COVID19(2023년), SARS, 변종, 진단, 치료제, [키워드] Activation Antiviral characterized Clinical outcome COVID-19 COVID-19 outbreak Defense develop drug Effect evade flexibility Genome human pathogen IL-18 IL-1β IL-6 immune immune response immunopathology implicated IMPROVE Infection infective Inflammasome inflammasome pathway inflammatory disease inhibiting Innate innate immune response innate immune system interferon Intervention mutant strain NLRP3 novel coronavirus SARS-CoV-2 pandemic pathology Predictive preventive measure pro-inflammatory cytokine promote protective role public health receptor Replication response responsible RNA virus SARS-CoV-2 SARS-COV-2 infection SARS-CoV2 shown the interferon treating COVID-19 Trigger urgent investigation vaccine candidate vaccine candidates vaccine for COVID-19 vaccine.. viral disease viral evolution virus viruses [DOI] 10.1007/s10753-022-01651-y PMC 바로가기
Metabolic and Proteomic Profiles Associated with Immune Responses Induced by Different Inactivated SARS-CoV-2 Vaccine CandidatesArticle Published on 2022-09-132022-11-15 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] acute respiratory syndrome Affect Amino acid association candidate cellular immunity change changed composed coronavirus COVID-19 creating Delta Epidemic immune immune responses immunized mice Inactivated vaccine Inactivated Vaccines induce induced metabolic metabolism metabolomic metabolomics mice neutralizing antibody positive profile Protein proteomic proteomics response resulting SARS-CoV-2 Vaccine vaccine candidates Vaccine design Vaccines Vaccines. variant variants Variation virus [DOI] 10.3390/ijms231810644 PMC 바로가기
Intranasal administration of a single dose of MVA-based vaccine candidates against COVID-19 induced local and systemic immune responses and protects mice from a lethal SARS-CoV-2 infectionArticle Published on 2022-09-122022-11-15 Journal: Frontiers in Immunology [Category] SARS, 변종, 진단, 치료기술, [키워드] absence acute respiratory syndrome Administered administration animal model blocked bronchoalveolar C57BL/6 caused CD4 CD8 coronavirus Coronavirus disease-19 COVID-19 COVID-19 progression current demonstrated effective Efficacy elicited evaluated expressing full-length histopathological IgA antibody IgG immune responses Immunity immunogenic immunogenicity induce intramuscular intranasal intranasal delivery K18-hACE2 transgenic mice Local lung lung lesion lymph nodes mice modified vaccinia virus morbidity and mortality mucosal mucosal immune MVA nasal washe Nasal washes neutralizing antibody parental Prevent pro-inflammatory cytokine PROTECT Protective protective efficacy Protein responses Result S protein SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 transmission SARS-CoV-2 variant SARS-CoV-2 virus SARS-CoV-2-specific immune response serum single dose spleen susceptible systemic immune response systemically T-cell immune response the SARS-CoV-2 Transmission undetectable upper respiratory tract vaccination Vaccine vaccine administration vaccine candidate vaccine candidates vaccine candidates. vaccine dose virus infection [DOI] 10.3389/fimmu.2022.995235 PMC 바로가기
Sialic Acid and Fucose Residues on the SARS-CoV-2 Receptor-Binding Domain Modulate IgG Antibody ReactivityArticle Published on 2022-09-092022-11-15 Journal: ACS Infectious Diseases [Category] SARS, 진단, [키워드] acid antibody antibody reactivity carbohydrate cells Chinese hamster conserved defined domain embryonic kidney cell ENhance evaluated exhibited fucose generate glycoepitope glycoproteomics IgG less mammalian cells mass spectrometry modulated monosaccharides N-linked glycans Neutralizing antibodies produced Protein RBD reactivity Receptor binding domain receptor binding domain. reduced removal SARS-CoV-2 SARS-CoV-2 positive patients serum sample sialic acid Spike protein Structure the RBD the RBD protein the SARS-CoV-2 vaccine candidates Viral proteins [DOI] 10.1021/acsinfecdis.2c00155 PMC 바로가기
Recombinant COVID-19 vaccine based on recombinant RBD/Nucleoprotein and saponin adjuvant induces long-lasting neutralizing antibodies and cellular immunityArticle Published on 2022-09-082022-11-15 Journal: Frontiers in Immunology [Category] SARS, 진단, 치료기술, [키워드] caused CD4+ CD4+ and CD8+ CD8+ T cell Cell cellular immunity Combination COVID-19 vaccine cytokine domain doses E. coli effective elicit elicited ELISA expressed female flow cytometry global pandemic granzyme groups IFN-γ IgG IgG1 IgG2a IL-12 IL-4 immune response immunized increase in induce infecting isotype long-lasting long-lasting neutralizing antibody lymphoproliferative mice N protein neutralization neutralization antibodies Neutralization antibody nucleocapsid percentage promoted Protective purified purified RBD quantified RBD recombinant Recombinant protein responses robust Saponin SARS-CoV-2 SARS-CoV-2 vaccine significantly spleen subcutaneously surrogate sVNT T cell response Test the SARS-CoV-2 Vaccine vaccine candidates vaccine group vaccine. virus were measured [DOI] 10.3389/fimmu.2022.974364 PMC 바로가기
Immunology to Immunotherapeutics of SARS-CoV-2: Identification of Immunogenic Epitopes for Vaccine DevelopmentArticle Published on 2022-09-052022-11-16 Journal: Current microbiology [Category] SARS, 유전자 메커니즘, [키워드] adaptive Antigen approach candidate caused Coverage COVID19 cytokine development epitope global public health homology identification immune in silico mechanism pandemic Pathogenesis pathway peptide Protein retrieved SARS-CoV-2 SARS-CoV-2 virus Signaling spike Stage T cell Trial Vaccine vaccine candidates Vaccine development virus [DOI] 10.1007/s00284-022-03003-3 PMC 바로가기
Identification of a novel immune-inflammatory signature of COVID-19 infections, and evaluation of pharmacokinetics and therapeutic potential of RXn-02, a novel small-molecule derivative of quinoloneResearch article Published on 2022-09-012022-10-05 Journal: Computers in biology and medicine [Category] COVID19(2023년), SARS, 변종, 신약개발, 치료기술, 치료제, [키워드] A549 activity acute respiratory syndrome addition Analysis AUC binding binding affinity Bioinformatics analysis candidate caused Cell cell line clinical investigation Colon conducted coronavirus Coronavirus disease 2019 COVID-19 COVID-19 (Coronavirus disease 2019) COVID-19 infections dataset drug drug target effector memory Efficacy evaluated exhibited FIVE gene signature global pandemic human lung IC50 identification immune immunomodulation immunomodulatory immunosuppressive in vitro in vivo Inflammation Inflammatory Lungs peak plasma concentration pharmacokinetics positive-sense RNA virus respiratory infection SARS-CoV-2 SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) SARS-CoV-2 drug SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 pathogenesis SARS-CoV-2-infected patient suggested T cell therapeutic options therapeutic potential therapy threat transcriptomic translation translational vaccine candidates value [DOI] 10.1016/j.compbiomed.2022.105814 [Article Type] Research article
Development of a pentavalent broadly protective nucleoside-modified mRNA vaccine against influenza B viruses Research Published on 2022-08-092022-10-05 Journal: Nature Communications [Category] 유전자 메커니즘, 치료제, [키워드] Antigen antigenic conserved cross-reactive immune response development effective elicit evaluate expressing Generation influenza B Influenza virus lineages long-lasting low dose messenger mice morbidity morbidity and mortality mRNA nucleoside-modified mRNA pentavalent platform PROTECT Protective reducing RNA single vaccination Support tested Vaccine vaccine candidate vaccine candidates vaccine platform virus virus [DOI] 10.1038/s41467-022-32149-8 [Article Type] Research